rabbit anti p eif 2α  (Cell Signaling Technology Inc)

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Poricoic acid A attenuates renal fibrosis by inhibiting endoplasmic reticulum stress-mediated apoptosis

doi: 10.1590/1414-431X2024e14249

Figure Lengend Snippet: Poricoic acid A (PAA) inhibited endoplasmic reticulum stress (ERS) activation in the kidney of unilateral ureteral obstruction (UUO) mice. A and B , Protein expression and quantitative analysis of Chop, GRP78, p-PERK, p-eif-2α, and ATF4. C , mRNA expression of Chop, GRP78, and ATF4. D , Immunohistochemical staining of Chop and GRP78 and relative quantitative analysis. Scale bar 25 μm. Data are reported as means±SD. *P<0.05, **P<0.01, ***P<0.001, and ns: not statistically significant. One-way ANOVA followed by a two-tailed Student's t -test. LOS: losartan.

Article Snippet: The primary antibodies included: collagen I (1:5000, ab34710, Abcam), fibronectin (1:1000, ab2413, Abcam), vimentin (1:2000, ab92547, Abcam), α smooth muscle actin (α-SMA,1:300, ab7817, Abcam), E-cadherin (1:2000, ab76055, Abcam), CHOP (1:1000, 2895, CST), GRP78 (1:1000, ab108615, Abcam), eIF-2α (1:1000, 9722, CST), P-eIF-2α (1:1000, 9721, CST), ATF4 (1:1000, AF2560, Beyotime), and α-tubulin (Proteintech).

Techniques: Activation Assay, Expressing, Immunohistochemical staining, Staining, Two Tailed Test

Journal: Fluids and Barriers of the CNS

Article Title: Pumilio-1 mediated translational control of claudin-5 at the blood-brain barrier

doi: 10.1186/s12987-024-00553-5

Figure Lengend Snippet: Histological localization of PUM1 in brain tissue from patients with epilepsy. ( a to f ) The expression level of CLDN-5 mRNA in resected human brain samples. The RNAs were isolated from the resected brain tissues obtained from control subjects ( n = 11) and patients with epilepsy ( n = 12). ( a ) CLDN-5, PUM1 and PUM2 mRNA expression levels between samples collected from control and epilepsy patients. CLDN-5 mRNA levels in the samples were broken down by ( b ) the difference of rs10314 genotypes (G is wild-type allele and C is rs10314 allele) and ( c ) seizure frequency. The data represents mean ± SD. ns, not significant ( p > 0.05); *p value ≤ 0.05; **p value ≤ 0.01. The correlation between CLDN-5 mRNA and ( d ) years of epilepsy, ( e ) PUM1 and ( f ) PUM2 mRNA levels. Spearman r values and P values are indicated. ( g ) Representative confocal images of human brain sections stained with anti-PUM1 antibody. A part of the hippocampal region showed a granular PUM1 staining pattern. Red; PUM1, Green; CLDN-5. Blue; nuclei. ( h ) The evaluation of the induction of stress granules in vascular endothelial cells in human brain sections (high CLDN-5). Sections were stained with anti-CLDN-5 (green) and stress granule marker, anti-p-eIF-2α or anti-PUM1 (red). Nuclei (blue) were counterstained with Hoechst

Article Snippet: The sections were incubated with mouse anti-CLDN-5 antibodies (clone 4C3C2, Thermo Fisher Scientific) and rabbit anti-PUM1 antibodies (clone EPR3795, Abcam) or rabbit anti- p-eIF-2α antibodies (44-728G, Thermo Fisher Scientific) in 1% NGS overnight at 4 °C.

Techniques: Expressing, Isolation, Staining, Marker